The Gadsden flag, proposing a rattlesnake and the phrase "Don't Tread on Me

https://andreaikh454.theglensecret.com/historic-civil-war-flags-understanding-their-role-inside-the-conflict

The Gadsden flag, offering a rattlesnake and the word "Don't Tread on Me," was once followed by way of a few Confederate items as a image of resistance opposed to perceived oppression.